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This repository doesn't have any active pull requests. Please file an issue or suggest a pull request. This repository doesn't have any active pull requests. Please file an issue or suggest a pull request. This repository doesn't have any active pull requests. Please file an issue or suggest a pull request.Fluorescence in situ hybridization Fluorescence in situ hybridization (FISH) is a technique in which the specific, labeled single-stranded DNA or RNA probe hybridizes to a complementary sequence, called the target sequence, inside the nucleus of the cell to be analyzed. Probes that are labeled with fluorescent molecules are designed to hybridize specifically to any DNA or RNA sequence. The probes can be labeled with fluorescent markers such as fluorescein (green), tetrachlorofluorescein (green-yellow), carboxytetramethylrhodamine (red), or digoxigenin (green). FISH is being used to probe sub-cellular localizations of DNA, RNA, proteins, metabolites, and other biomolecules. History The technique was first described by Arno G. Seeger in 1959, while studying the metaphase chromosomes of the sea urchin in the green fluorescent light. The technique was based on the principle of fluorescence, where the excitation of a fluorophore causes it to emit a distinct wavelength of light, which would reveal the exact place of the target DNA in the cell. Probes can be detected using fluorescent microscopes or image analysis. Applications FISH is a sensitive method for detecting DNA and RNA. DNA FISH (e.g., FISH4) DNA FISH involves a combination of DNA labeling (using a sequence specific dye) and fluorescent microscope. The probes are labeled with a fluorescent dye (e.g., Cy3, fluorescein, tetrachlorofluorescein, digoxigenin, or biotin) and attached to a sequence-specific oligonucleotide probe. It is a robust and reliable technique for mapping the chromosomal location of a DNA sequence. Transcribed FISH (e.g., RNA FISH) Transcribed FISH (or fluorescent in situ hybridization or FISH) is a method to determine the location of

 

 

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